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BIO - AFM (CRF Shared Facility) IIT Delhi

Atomic force microscope (AFM) is a type of scanning probe microscopes (SPM). SPMs are designed to measure local properties, such as height, friction, magnetism, through a moving probe. To acquire an image, the probe scans over a small area of the sample and measures the local property such as topology and roughness simultaneously. Basically, an AFMs operates by measuring the force between a probe and the sample. Although the lateral resolution of AFM is low (~30nm) due to the convolution, the vertical resolution can be up to 0.1nm. The BioScope Catalyst Atomic Force Microscope (AFM) is designed specifically for safety and reliability when working with biological samples. It is capable for broad range of imaging and material property characterization of protein based samples, wide range of cell types including human, other vertebrates, fungal, algal and bacterial as well as chemically synthesised scaffolds with high accuracy.

Features of BIO AFM
Sample requirment for BIO - AFM (General Instructions for Users)
  1. Samples for Bio-AFM analysis need to meet certain requirements for obtaining good imaging attributes. The more careful you prepare samples the more likely a successful analysis will be. Here are some guidelines of which kind of treatment is advantageous for Bio-AFM analysis and which is not:
  2. The Bio-AFM cantilevers are highly brittle and may break during imaging of very stiff and high material deposition. It is recommended only for soft and biological origin samples. Various contaminants such as PEG, keratin and high salt concentrations in samples are not recommended. Scalpel, pipette tips and tubes should be clean. Please use fresh Eppendorf tubes.
  3. High resolution imaging and surface analysis of lipid monolayers and bilayers.
  4. Measurement of nanomechanical material properties such as surface roughness and elasticity.
  5. Samples can be submitted in solid, liquid, or solution form. If you are submitting a sample in solution, you should indicate the solvent on the submission form that your sample is dissolved in and other solvents in which your sample is soluble. Suitable solvents should also be provide along with the samples.
  6. For sample work up and purification fresh HPLC grade solutions and reagents should be used.
  7. Please supply the sample in a low-salt buffer for the obtaining clarity during imaging and improved interpretation.
  8. Relevant literature and protocols (in case specific to certain sample types) should be supplied before hand and associated components (buffers) should also be provided.
Click here to Get 
BIO AFM Charge List

How to Contact

Physical Location:

Bio-AFM Laboratory 
Besides JEE-JAM offices, Block 1 
IIT Delhi,  Hauz Khas, New Delhi – 110016 
Phone number : 011-2659-7397

Coordinator:

Prof. Bishwajit Kundu 
Kusuma School of Biological Sciences IIT- Delhi, 
Hauz Khas, New Delhi – 110016 
Phone : +91-11-2659-1037 
Email : bkundu@bioschool.iitd.ac.in

Operational Incharge

Dr  Naminita Gogoi
MG-2 Mass Spectrometry Laboratory 
Ground Floor, 
Kusuma School of Biological Sciences 
IIT-Delhi, Hauz Khas, New Delhi – 110016
Phone number : 011-2659-7503 
Email : ird12412@bioschool.iitd.ac.in

Get CRF Registration for 
External users 

1. Registration process (One Time): New Registration for external users.User Verification: Upload photo of Front side and back side of valid Identity card issued by the organization (only .jpeg format, file Size less than 1 Mb, height less than 1000 pixels, width less than 800 pixels). Undertaking: Download the undertaking template ( Download Link ). Duly filled, signed and stamped form should be uploaded in .pdf format. Confirmation: An email will be sent to the user after verification of the documents.

2.Before planning to avail the Instrument facility of CRF IIT Delhi: user must visit https://crf.iitd.ac.in -> Facility -> select the desired facility from left side navigation list.  Read carefully all the relevant information such as required sample type, testing charges and instrument location. Be prepared with details of the samples.

3.Log in and Click on Booking Appointment Tab: All the information relevant to testing must be filled in instrument booking form. In case user does not provide the required information the booking is liable to be rejected. User can upload the relevant existing data of their samples that might help in the measurements.

4.Sample submission & Payment Process: Once the application is submitted, the in-charge will verify the details and approve the request.External users must wait for the approval before making the payment and submitting the samples to concerned lab. After approval the payment should be made by the user. The payment transaction details need to be submitted online before appointment date time.

5.CRF Users can pay amount online using following details:
Beneficiary/Customer's Name: IRD ACCOUNT IITD
Bank Account Number:  10773572600  
Bank Name: State Bank of India Branch Name: IIT Branch
Branch Code: 01077
NEFT IFSC CODE / RTGS: SBIN0001077
please write "CRF _Instrument Name" in the Remarks section while paying. Save the receipt file and upload in the crfbooking system online against your application.Payment can also made in form of demand draft in favour of " IRD ACCOUNT IITD " Payable at New Delhi.6.IIT Delhi 
GST Number : 07AAATI0393L1ZI
PAN Number : AAATI0393L

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