Electrospray ionization (ESI) is a soft ionization technique to examine proteins using MS and MS/MS. In ESI, charged droplets are produced by passing a solubilized sample through a high voltage needle at atmospheric pressure. Desolvation occurs prior to entrance into the high vacuum of the mass spectrometer. This approach is often coupled with a chromatographic system, typically reverse phase chromatography or capillary electrophoresis, allowing analysis of very complex samples. ESI typically induces a range of charge states. LC-MS is a powerful technique used for many applications which has very high sensitivity and selectivity.
The ESI-MS facility at KSBS, IIT Delhi is a Bruker Electrospray Ionization instrument-amaZon SL Dual Funnel Iontrap bench top with a mass accuracy of 0.1Da. It is connected to a nano-liquid chromatography setup- EASY nLC-II with C18 column to fractionate sample containing mixture of peptides. There is a facility of direct probe injection as well as sample injection through nLC. This instrument is capable of performing analysis of intact proteins, mixtures of proteins and peptides, biomarker studies, identification of post translational modifications, and many more applications.
Mass Spectrometer Features:
1. Dual Ion Funnel technology delivers superior sensitivity in the low femtogram range
2. Fast data acquisition with scan speeds up to 32,000 u/s at full isotopic resolution
3. Reproducible spectra for high-confidence MS/MS library searches
Sample Preparation Guidelines Samples :
1. Registration process (One Time): New Registration for external users.User Verification: Upload photo of Front side and back side of valid Identity card issued by the organization (only .jpeg format, file Size less than 1 Mb, height less than 1000 pixels, width less than 800 pixels). Undertaking: Download the undertaking template ( Download Link ). Duly filled, signed and stamped form should be uploaded in .pdf format. Confirmation: An email will be sent to the user after verification of the documents.
2.Before planning to avail the Instrument facility of CRF IIT Delhi: user must visit https://crf.iitd.ac.in -> Facility -> select the desired facility from left side navigation list. Read carefully all the relevant information such as required sample type, testing charges and instrument location. Be prepared with details of the samples.
3.Log in and Click on Booking Appointment Tab: All the information relevant to testing must be filled in instrument booking form. In case user does not provide the required information the booking is liable to be rejected. User can upload the relevant existing data of their samples that might help in the measurements.
4.Sample submission & Payment Process: Once the application is submitted, the in-charge will verify the details and approve the request.External users must wait for the approval before making the payment and submitting the samples to concerned lab. After approval the payment should be made by the user. The payment transaction details need to be submitted online before appointment date time.
5.CRF Users can pay amount online using following details:
Beneficiary/Customer's Name: IRD ACCOUNT IITD
Bank Account Number: 10773572600
Bank Name: State Bank of India Branch Name: IIT Branch
Branch Code: 01077
NEFT IFSC CODE / RTGS: SBIN0001077
please write "CRF _Instrument Name" in the Remarks section while paying. Save the receipt file and upload in the crfbooking system online against your application.Payment can also made in form of demand draft in favour of " IRD ACCOUNT IITD " Payable at New Delhi.6.IIT Delhi
GST Number : 07AAATI0393L1ZI
PAN Number : AAATI0393L
1 Registration process (One Time): New Registration for internal users.
Fill in all the details in Personal Information, Supervisor Information and User Login Credentionals.
Once submitted, an email to supervisor is sent for activation of account. Once activated by supervisor, you can do the instrument bookings.
2 Before planning to avail the Instrument facility of CRF IIT Delhi: user must visit https://crf.iitd.ac.in > Facilities > select the desired facility from left side navigation list. Read ca 
refully all the relevant information such as required sample type, testing charges and instrument location. Be prepared with details of the samples.
3. Log in on CRF Booking System and Click on Booking Appointment Tab: All the information relevant to testing must be filled in instrument booking form. In case user does not provide the required information the booking is liable to be rejected. User can upload the relevant existing data of their samples that might help in the measurements.
4 Sample submission & Payment Process: Once the application is submitted, the in-charge will verify the details and approve the request.
