CRF Intranet

FACS (Cell Biology Lab Sonipat Campus)

The Flow Cytometry BD FACSAria-III is the high quality, multi-parameter flow cytometry analyses and sorting instrument. The Facility is capable of cell sorting and analysis (sterile, at speeds up to 50,000 cells/sec) from homogeneous or mixed cell populations based on up to 14 fluorochromes, sorting up to four separate populations simultaneously and cell sorters can also sort any number of cells into each well of a 6, 24, 48, 96, or 384-well plate. Any suspended particle or cell from 0.5–70 micrometres in size is suitable for sorting and analysis. Cells from solid tissue must be disaggregated into single cell suspension before analysis/sorting.

Violet Laser - 405nm
PMT Band Pass Long Pass Fluorochrome
A 780/60 750LP BV786
B 710/50 610LP BV711
C 660/20 630LP BV605
D 610/20 595LP BV605
E 510/50 505LP BC510 , V500, BV480
F 450/40 - BV425 , Pacific Blue, V450
G - - -
H - - -
Blue Laser - 488nm
PMT Band Pass Long Pass Fluorachrome
A 695/40 655LP PerCP
B 530/30 502LP PerCP - Cy5
C 488/10 - FITC, GFP
Yellow Green Laser - 561 nm
PMT Band Pass Long PAss Fluorochrome
A 780/90 735LP PE-Cy7
B 710/50 685LP PE-Cy5.5
C 670/14 630LP PE-Cy5
D 610/20 600LP PE-CEF94
E 582/15 - PE
F - - -
G - - -
H - - -
Red Laser - 633nm
PMT Band PAss Long PAss Fluorochrome
A 695/40 655LP APC- Cy7, ACP-H7
B 530/30 502LP Alexa Fluor 700
C 488/10 - ACP , Alexa Fluor 647
Sorting and Analysis Sample

The safety of the staff and users of the facility is the ultimate concern when sorting unfixed samples containing unscreened human or nonhuman primate cells, known infectious agents (≥ risk group 2), or recombinant or synthetic nucleic acid molecules or vectors. Therefore, information about the sample source and potentially bio hazardous agents is critical for effective biosafety risk assessment.

Appropriate biosafety approval by the IITD and the core facility is required prior to the use of the machines.

Please provide this Sort/Analysis Sample Request Form to the FACS Core upon scheduling a sort. This form must be filled out completely and signed by the principal investigator for each sort requested, even for analysis. Without the proper risk assessment enabled by this questionnaire and supporting information, bookings will NOT be approved.

Refer to web site: https://isac-net.org/page/Biosafety for available Instrument.

The International Society for Advancement of Cytometry revised its Cell Sorter Biosafety Standards in 2014. J. of the International Society for the Advancement of Cytometry, Cell Sorter Biosafety Standards, 2014. This table provides a concise summary of recommended bio-containment levels for cell sorting and analysis.

Cell Type , Source, Experimental Condition or Modification and Bio-containment for Flow Sorting 
Cell Type and Unfixed Source, Experimental Condition or Modification Bio-containment for Flow Sorting
Mouse (or other non primate) Transduced with replication-deficient lentivirus or retrovirus vector (and vector stock tested negative for replication competent virus) BSL2
Mouse (or other non primate) Transduced with replication-competent lentivirus or retrovirus vector or Transduced with replication-deficient lentivirus or retrovirus vector (and vector stock NOT tested for replication competent virus) BSL2 + hood
Human or non-human primate Human cell lines (regardless of source, or Testing for pathogens). BSL2 + hood
Human or non-human primate Includes all primary cells (regardless of source, or testing for pathogens). Includes cells from humanized mice. BSL2-enhanced
Any Infected with risk-group 2 agents requiring BSL-2 containment. Examples: LCMV, Vaccinia, Listeria, Malaria, KSHV, Aspergillus, Cryptococcus, etc BSL2-enhanced
Any Infected with agents requiring BSL-3 containment. Example: Mycobacterium tuberculosis BSL-3
CRF Testing Charge List
How to Contact

Coordinator

Prof. Shilpi Sharma
Biochemical Engineering & Biotechnology,
IIT Delhi, New Delhi 110016 
Email: shilpi@dbeb.iitd.ac.in

Coordinator

Prof. Amitabha Mukhopadhyay 
Kusuma School of Biological Sciences, IIT-Delhi, Haus Khas New delhi-110016  
Email: amitabha.mukhopadhyay@bioschool.iitd.ac.in

Coordinator

Prof. James Gomes 
Kusuma School of Biological Sciences, IIT-Delhi, Haus Khas 
New delhi-110016 
Email: jgomes@bioschool.iitd.ac.in

Coordinators

Prof. Neetu Singh 
Center for Biomedical Engineering  Block-II, 
Room No. 388
Indian Institute of Technology Delhi,  Hauz Khas,
New Delhi-110016 
Email: sneetu@iitd.ac.in

Mailing Address for Samples & Lab Location

Cell Biology Lab, Room No. 552, 3rd Floor IIT-Delhi, Sonipat Campus Rajiv Gandhi Education City Rai Industrial Area, 
Sonipat-131029, Haryana.

Operational Scientist/Incharge

Dr. Vikash Kumar, PhD
Principal Project Scientist,
CRF, IIT-Delhi, Sonipat Campus
Rajiv Gandhi Education City
Rai Industrial Area, Sonipat-131029, Haryana.
Email: ​vikash2.cstaff@bioschool.iitd.ac.in

Get CRF Registration for 
External users 

1. Registration process (One Time): New Registration for external users.User Verification: Upload photo of Front side and back side of valid Identity card issued by the organization (only .jpeg format, file Size less than 1 Mb, height less than 1000 pixels, width less than 800 pixels). Undertaking: Download the undertaking template ( Download Link ). Duly filled, signed and stamped form should be uploaded in .pdf format. Confirmation: An email will be sent to the user after verification of the documents.

2.Before planning to avail the Instrument facility of CRF IIT Delhi: user must visit https://crf.iitd.ac.in -> Facility -> select the desired facility from left side navigation list.  Read carefully all the relevant information such as required sample type, testing charges and instrument location. Be prepared with details of the samples.

3.Log in and Click on Booking Appointment Tab: All the information relevant to testing must be filled in instrument booking form. In case user does not provide the required information the booking is liable to be rejected. User can upload the relevant existing data of their samples that might help in the measurements.

4.Sample submission & Payment Process: Once the application is submitted, the in-charge will verify the details and approve the request.External users must wait for the approval before making the payment and submitting the samples to concerned lab. After approval the payment should be made by the user. The payment transaction details need to be submitted online before appointment date time.

5.CRF Users can pay amount online using following details:
Beneficiary/Customer's Name: IRD ACCOUNT IITD
Bank Account Number:  10773572600  
Bank Name: State Bank of India Branch Name: IIT Branch
Branch Code: 01077
NEFT IFSC CODE / RTGS: SBIN0001077
please write "CRF _Instrument Name" in the Remarks section while paying. Save the receipt file and upload in the crfbooking system online against your application.Payment can also made in form of demand draft in favour of " IRD ACCOUNT IITD " Payable at New Delhi.6.IIT Delhi 
GST Number : 07AAATI0393L1ZI
PAN Number : AAATI0393L

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