CRF Intranet

Fluorimeter  (CRF Shared facility ) DBEB Instrumntation Lab

Fluorimeter is used to detect the fluorescence light, emitting from the sample after its continuous irradiation with beam of light. Properties of molecules like its dynamics and interaction with other molecules can be studied using Fluorimeter. Fluorimeter can also be used to study different fluorescence properties like resonance energy transfer and quenching. Fluorescence intensity gives time averaged information of fluorophore present in excited state. Variations in fluorescence intensity and emission maximum can be utilized to studies many important biological phenomena. Fluorescence spectrum can also reveal information about the solvent exposure and degree of polarity around the fluorophore. Fluorescence spectroscopy can detects very low concentration and it is about 10,000 times more sensitive than absorption spectroscopy.

Fluorimeter is equipped with well-plate reader, which can read up to 96 well plates in either the X or Y direction.

Sample Conditions for Fluorimeter

1) We need minimum 1 mL completely dissolved liquid sample.
2) Absorbance (Optical Density) at Excitation wavelength of the sample should be around 0.04.
3) We will process your samples once we got order for minimum 20 samples.
4) We will provide you raw data of single point emission.

Before CRF Booking for Fluorimeter emission analysis instrument booking user must know the about the required parameters of samples for testing.
  1. Excitation wavelength
  2. Emission wavelength (for single point emission):
  3. Excitation slit width
  4. Emission slit width:
  5. Emission Filter (290/350/390/430/515 nm Cut-off):
List of CRF Charges

How to Contact 

Mailing Address for Samples & Lab Location 

Instrument Lab Block I, 
Room No. 233 Department of Biochemical Engineering and Biotechnology 
Indian Institute of Technology Hauz Khas, 
New Delhi-110016, India

Operational Incharge 


Dr. Ziauddin Ansari, 
Senior Lab Assistant, 
DBEB Block I, 
Room No 233 Department of Biochemical Engg. and Bio Technology 
IIT Delhi Hauz Kash New Delhi 110016
Phone No.: (011) 2659-6151

Get CRF Registration for 
External users 

1. Registration process (One Time): New Registration for external users.User Verification: Upload photo of Front side and back side of valid Identity card issued by the organization (only .jpeg format, file Size less than 1 Mb, height less than 1000 pixels, width less than 800 pixels). Undertaking: Download the undertaking template ( Download Link ). Duly filled, signed and stamped form should be uploaded in .pdf format. Confirmation: An email will be sent to the user after verification of the documents.

2.Before planning to avail the Instrument facility of CRF IIT Delhi: user must visit https://crf.iitd.ac.in -> Facility -> select the desired facility from left side navigation list.  Read carefully all the relevant information such as required sample type, testing charges and instrument location. Be prepared with details of the samples.

3.Log in and Click on Booking Appointment Tab: All the information relevant to testing must be filled in instrument booking form. In case user does not provide the required information the booking is liable to be rejected. User can upload the relevant existing data of their samples that might help in the measurements.

4.Sample submission & Payment Process: Once the application is submitted, the in-charge will verify the details and approve the request.External users must wait for the approval before making the payment and submitting the samples to concerned lab. After approval the payment should be made by the user. The payment transaction details need to be submitted online before appointment date time.

5.CRF Users can pay amount online using following details:
Beneficiary/Customer's Name: IRD ACCOUNT IITD
Bank Account Number:  10773572600  
Bank Name: State Bank of India Branch Name: IIT Branch
Branch Code: 01077
NEFT IFSC CODE / RTGS: SBIN0001077
please write "CRF _Instrument Name" in the Remarks section while paying. Save the receipt file and upload in the crfbooking system online against your application.Payment can also made in form of demand draft in favour of " IRD ACCOUNT IITD " Payable at New Delhi.6.IIT Delhi 
GST Number : 07AAATI0393L1ZI
PAN Number : AAATI0393L

Signup (External user)

CRF IIT Delhi 
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