CRF Intranet

SPRS(Surface Plasmon Resonance Spectroscope)(Cell -Biology Lab Sonipat Campus) 

Surface Plasmon Resonance Spectroscope: Biacore™ systems from GE Healthcare exploit the phenomenon of surface plasmon resonance (SPR) to monitor the interaction between molecules in real time. It involves attachment of one interacting partner to the surface of a sensor chip, and then passing sample containing other (potential) interaction partners over the surface. Binding of molecules to the sensor surface generates a response which is proportional to the bound mass, and changes in amount bound can be detected down to a few picograms per square millimeter on the sensor surface. Binding events are followed in real time and a range of interaction characteristics can be determined.

Company Name: GE Healthcare Life Sciences (Now Cytiva) Model: Biacore X100
Among the questions that can be answered with Biacore are:
Sample Preparation Guidelines: 
Kindly note that USERS must bring their own CD-R/CD-RW for data collection and storage including any desired back-up.
  1. SPR is extensively employed to study protein-protein, antigen-antibody and small molecule ligand-receptor interactions. The protein is immobilized on the sensor chip and the ligand molecules are flown over it to study the effective binding affinities and kinetic constants.
  2. The user must bring their own sensor chip, ligand and analyte samples, buffers (immobilization, running and regeneration) and an unopened CD-R/CD-RW as well as all other required solvents and consumables according to the need of their experiment. No consumable will be provided by the operator incharge.
CRF Testing Charge List
How to Contact

Coordinator

Prof. Shilpi Sharma
Biochemical Engineering & Biotechnology,
IIT Delhi, New Delhi 110016 
Email: shilpi@dbeb.iitd.ac.in

Coordinator

Prof. Amitabha Mukhopadhyay 
Kusuma School of Biological Sciences, IIT-Delhi, Haus Khas New delhi-110016  
Email: amitabha.mukhopadhyay@bioschool.iitd.ac.in

Coordinator

Prof. James Gomes 
Kusuma School of Biological Sciences, IIT-Delhi, Haus Khas 
New delhi-110016 
Email: jgomes@bioschool.iitd.ac.in

Coordinators

Prof. Neetu Singh 
Center for Biomedical Engineering  Block-II, 
Room No. 388
Indian Institute of Technology Delhi,  Hauz Khas,
New Delhi-110016 
Email: sneetu@iitd.ac.in

Mailing Address for Samples & Lab Location

Cell Biology Lab, Room No. 552, 3rd Floor IIT-Delhi, Sonipat Campus Rajiv Gandhi Education City Rai Industrial Area, 
Sonipat-131029, Haryana.

Operational Scientist/Incharge

Dr. Vikash Kumar, PhD
Principal Project Scientist,
CRF, IIT-Delhi, Sonipat Campus
Rajiv Gandhi Education City
Rai Industrial Area, Sonipat-131029, Haryana.
Email: ​vikash2.cstaff@bioschool.iitd.ac.in

Get CRF Registration for 
External users 

1. Registration process (One Time): New Registration for external users.User Verification: Upload photo of Front side and back side of valid Identity card issued by the organization (only .jpeg format, file Size less than 1 Mb, height less than 1000 pixels, width less than 800 pixels). Undertaking: Download the undertaking template ( Download Link ). Duly filled, signed and stamped form should be uploaded in .pdf format. Confirmation: An email will be sent to the user after verification of the documents.

2.Before planning to avail the Instrument facility of CRF IIT Delhi: user must visit https://crf.iitd.ac.in -> Facility -> select the desired facility from left side navigation list.  Read carefully all the relevant information such as required sample type, testing charges and instrument location. Be prepared with details of the samples.

3.Log in and Click on Booking Appointment Tab: All the information relevant to testing must be filled in instrument booking form. In case user does not provide the required information the booking is liable to be rejected. User can upload the relevant existing data of their samples that might help in the measurements.

4.Sample submission & Payment Process: Once the application is submitted, the in-charge will verify the details and approve the request.External users must wait for the approval before making the payment and submitting the samples to concerned lab. After approval the payment should be made by the user. The payment transaction details need to be submitted online before appointment date time.

5.CRF Users can pay amount online using following details:
Beneficiary/Customer's Name: IRD ACCOUNT IITD
Bank Account Number:  10773572600  
Bank Name: State Bank of India Branch Name: IIT Branch
Branch Code: 01077
NEFT IFSC CODE / RTGS: SBIN0001077
please write "CRF _Instrument Name" in the Remarks section while paying. Save the receipt file and upload in the crfbooking system online against your application.Payment can also made in form of demand draft in favour of " IRD ACCOUNT IITD " Payable at New Delhi.6.IIT Delhi 
GST Number : 07AAATI0393L1ZI
PAN Number : AAATI0393L

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